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By Alton Meister

Advances in Enzymology and comparable components of Molecular Biology is a seminal sequence within the box of biochemistry, supplying researchers entry to authoritative studies of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, supplying an unequalled view of the ancient improvement of enzymology. The sequence deals researchers the most recent knowing of enzymes, their mechanisms, reactions and evolution, roles in advanced organic strategy, and their software in either the laboratory and undefined. each one quantity within the sequence gains contributions by way of major pioneers and investigators within the box from all over the world. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.

With its wide variety of subject matters and lengthy ancient pedigree, Advances in Enzymology and similar components of Molecular Biology can be utilized not just via scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition by means of any scientist attracted to the invention of an enzyme, its houses, and its purposes.

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11. Bumstead, R. , Dahl, J. , and Strominger, J. , J. Biol. , 243, 770 (1968). 12. Stewart, T. , Roberts, R. , and Strominger, J. , Nature, 230, 36 (1971). 13. Mandel, L. , Biochemistry, 2, 560 (1963). 14. I. , Biochem. Biophys. Res. ,36, 435 (1969). 15. , E’ed. , 24, 292 (1965). 16. Pillinger, D. , Hay, J . , Biochem. J . , 114, 429 (1969). 17. , Progr. in Nucleic Acid Res. and Mol. , l I , 391 (1971). 18. , and Boman, H. , Proc. N u t . Acacl. Sci. U S . , 53, 134 (1965). 19. Phillips, J. , J .

Can. J . , 47, 561 (1969). 67. , Abstr. Amer. Soc. Biol. Chem. (1971). 68. , Boman, H. , J . Mol. , 9 , 831 (1964). 69. Lowrie, R. , and Bergquist, P. , Biochemistry, 7 , 1761 (1968). 7 0 . , 18, 1209 (1958). 71. Haneock, R. , Biochem. Biophys. Res. , 31, 77 (1968). 72. , and Littauer, U . , Biochemistry, 6 , 451 (1967). 73. Kaye, A. , and Leboy, P. , Biochem. Biophys. Acta, 157, 289 (1968). 74. McFarlane, E. , Can. J . , 15, 189 (1969). 75. Leboy, P. , Biochemistry, 9 , 1577 (1970). THE t R N A METHYLTRANSFERASES 27 76.

COVALENT LINKING REACTIONS 1. Cellulose and Polystyrene Perhaps the most common covalent linkage of enzymes and antigens to cellulose and polystyrene derivatives is by the reaction of histidyl and tyrosyl residues of these proteins with diazotized derivatives of the matrix. Pressman e t al. (36) were the first to utilize this technique in binding ovalbumin to sheep erythrocytes using bisdiazobenzidine as a divalent coupling agent. Campbell e t al. (9,37)subsequently characterized immunoadsorbents prepared by coupling ovalbumin to diazotized p-aminobenzyl cellulose in detail.

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