Download NMDA Receptor Protocols by Fumio Nakamura, Yoshio Goshima, Stephen M. Strittmatter PDF

By Fumio Nakamura, Yoshio Goshima, Stephen M. Strittmatter (auth.), Min Li (eds.)

In NMDA Receptor Protocols, Min Li and a panel of hands-on experimentalists element cutting-edge molecular concepts for learning NMDA ligand-gated ion channels and constructing assays for nontherapeutic lead choice. the themes diversity from cDNA cloning to in vitro and in vivo research of the channel complicated within the mammalian mind. extra issues contain the biochemical research of the channel protein and the development of varied heterologous platforms for either easy examine and excessive throughput displays (HTS) for pharmaceutical chemical substances. even though the point of interest is on NMDA receptors, the equipment are appropriate to different ligand-gated ion channels and with a few amendment can be prolonged to comparable membrane receptors or signaling receptors.
NMDA Receptor Protocols offers brand new scientists in any respect ability degrees a various choice of hugely reproducible molecular options for ion channel examine and biotherapeutic improvement. Eminently useful and reproducible, those innovations provide strong tools for uncomplicated learn on NMDA receptor constitution and serve as, in addition to huge, immense possibilities for medical research towards the improvement of novel bioactive compounds.




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Mol. Cell. Neurosci. 2, 320–330. 39. , et al. (1993) Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits. J. Biol. Chem. 268, 2836–2843. 40. , Laurie, D. , and Seeburg, P. H. (1994) Developmental and regional expression in the rat brain and functional properties of four NMDA receptors. Neuron 12, 529–540. Functional NMDA Receptors 33 3 Transient Expression of Functional NMDA Receptors in Mammalian Cells Paul L. Chazot, Miroslav Cik, and F. Anne Stephenson 1. Introduction The NMDA subtype of excitatory glutamate receptor is a multisubunit, fastacting, ligand-gated cation channel with a high permeability for Ca2+.

There is mounting evidence that NMDA receptors are heteromeric complexes composed of NMDAR1 and NMDAR2 (A, B, C, or D) subunits (4). Each of the mRNAs encoding these subunits has a distinct anatomical distribution (5), and coexpression of different combinations of these subunits results in channels with unique biophysical and pharmacological properties (6). Subtype-selective agonists, antagonists, and modulators of NMDA receptors can be valuable pharmacological tools in understanding the physiological roles of specific NMDA receptor subtypes.

1994) Single-channel properties of cloned NMDA receptors in a human cell line: comparison with results from Xenopus oocytes. J. Physiol. 476, 391–397. Chazot, P. , and Stephenson, F. A. (1992) Immunological detection of the NMDAR1 glutamate receptor expressed in human embryonic kidney 293 cells and in rat brain. J. Neurochem. 59, 1176–1178. Chazot, P. L. and Stephenson, F. A. (1997) Biochemical evidence for the existence of a pool of unassembled C2 exon-containing NR1 subunits of the mammalian forebrain NMDA receptor.

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